Rapamycin Cream

rapamycin cream is a topical therapy for senescent skin cells that accumulate over time and contribute to the appearance of wrinkles and age spots. Infused with EGCG to enhance skin absorption, the formulation provides targeted treatment for aging skin, improving its appearance and health.

Senescent cells produce pro-inflammatory cytokines, matrix metalloproteins and reduced levels of anti-angiogenic factors. These changes, called the senescence-associated secretory phenotype (SASP), create a harmful environment that is permissive for tumor formation. Hence, senescent cells are key contributors to aging and disease in humans.

Aging is a biological process that leads to cellular dysfunction and the loss of organ function. The underlying causes of this process include increased rates of cell death and the accumulation of pro-inflammatory senescent cells in tissues.

The rapamycin compound sirolimus has been shown to slow the progression of these programs in mice. By reducing mTOR activity, it extends the lifespan of these animals and delays diseases. It has been approved for use in a variety of clinical settings, including transplant patients to prevent allograft rejection and lymphangiomatosis.

Topical rapamycin is a safe and effective treatment for senescent skin cells that accumulate in the epidermis. The peptide reduces the total number of senescent cells and improves skin elasticity by decreasing p16 protein, a marker for aging in the skin.

A gel-based rapamycin formulation was developed to improve skin penetration using various excipients to promote tolerance to the drug. Its stability was evaluated after 1 year at 4degC to determine if it could be used as an anti-aging therapy for skin.

Physical and microbiologic stability of the gels was measured by evaluating their organoleptic characteristics, pH, rapamycin concentration, size and repartition of a-tocopherol droplets. The gels were also inspected visually for their appearance and consistency.

Rapamycin was stable for at least one year under conditions of temperature, humidity and agitation in the presence of polyethylene glycol. However, the concentration of rapamycin and tocopherol droplet size did not significantly decrease during this period.

Stability and the rapamycin-tocopherol complex are important properties that must be taken into account when developing a rapamycin formulation for the prevention of senescent cells. Moreover, the rapamycin-tocopherol compound should be physically and microbiologically stable to ensure that it can be applied to skin over an extended period of time.

This study is the first to examine the effect of a rapamycin-tocopherol gel on the stability and rheological behaviour of the hydro-alcoholic rapamycin compound in a controlled environment. The gel-based formulation was compared to a lipophilic ointment in terms of rapamycin content, the soluble state and the optimum pH for cutaneous absorption.

The results from this study provide a clear indication that the rapamycin-tocopherol combination is stable over an extended period of time in a gel-based form. It also indicates that rapamycin is not easily degraded and can be used as an anti-aging drug for skin, due to its ability to delay the development of senescent cells and prevent oxidative damage in the epidermis.